Pluripotency of a Single Spermatogonial Stem Cell in Mice1
نویسندگان
چکیده
منابع مشابه
Expression of Spermatogonial and Pluripotency Markers in Spermatogonial Stem Cells after Treatment with Different Culture Factors
Background: As condition and component of culture determine fate map of spermatogonial stem cells (SSCs), the aim of this study was to evaluate of growth factors GDNF, LIF and RA on proliferation and differentiation of SSC. Materials and Methods: SSCs were cultured in two groups: The first group GDNF and LIF and the second group RA. The number of clumps and colony formation was monitored dur...
متن کاملIn Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direct induction of ESC-like cells from well-characterized SSCs. Thus, the aim of t...
متن کاملexpression of spermatogonial and pluripotency markers in spermatogonial stem cells after treatment with different culture factors
background: as condition and component of culture determine fate map of spermatogonial stem cells (sscs), the aim of this study was to evaluate of growth factors gdnf, lif and ra on proliferation and differentiation of ssc. materials and methods: sscs were cultured in two groups: the first group gdnf and lif and the second group ra. the number of clumps and colony formation was monitored during...
متن کاملSpermatogonial Stem Cell Niche and Spermatogonial Stem Cell Transplantation in Zebrafish
BACKGROUND Spermatogonial stem cells (SSCs) are the foundation of spermatogenesis, and reside within a specific microenvironment in the testes called "niche" which regulates stem cell properties, such as, self-renewal, pluripotency, quiescence and their ability to differentiate. METHODOLOGY/PRINCIPAL FINDINGS Here, we introduce zebrafish as a new model for the study of SSCs in vertebrates. Us...
متن کاملDefining the spermatogonial stem cell.
Through the use of donor cells from transgenic rats expressing GFP exclusively in the germline, we have defined culture conditions where male germ cells lose (on STO cells) or maintain (on MSC-1 cells) stem cell activity. A cadre of germ cell transcripts strikingly decrease in relative abundance as a function of testis age or culture time on STO cells, but only a subset of these transcripts (ap...
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ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 2008
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod.107.066068